Zwoinska et al 2020 Heat-induced sterility data

Summary:

This data was used to assess how sublethal heat stress during development impacted subsequent adult fertility and its plasticity, both of which can provide the raw material for evolutionary responses to increased temperature. We quantified phenotypic (and genetic) variation in fertility of Drosophila melanogaster reared at standardized densities in three temperatures (25, 27, 29°C) from a set of lines of the Drosophila Genetic Reference Panel (DGRP).



Files available:

We provide one excel (with six tabs) and 6 csv files. The data in the excel is the same as the one provided in the 6 csv files.


TableS1_archival_data.xlsx

This data file has six tabs, each tab associated with a dataset used in the analysis.


Tab: Male data raw (= Zwoinska20_malerawdata.csv)

This tab contains the raw data of male fertility at three different developmental temperatures.

Table headers are explained below:

Batch = block

DGRP = DGRP line

Temperature = developmental temperature experienced

Vial = vial where each pair was kept

Larvae = 1, if larvae present; 0, if no larvae present


Tab: Male data (= Zwoinska20_maledata.csv)

This tab contains compacted data per DGRP line from the male fertility assay at three different developmental temperatures, including inversions and intercepts + slopes extracted from statistical models. Table headers are explained below:

DGRP Line = line number

LineNo = abbreviated line number

Reproduced 25°C = all vials in that block (batch) which at least one larva was found; same explanation for columns "Reproduced 27" and "Reproduced 29"

All 25°C = total number of males (vials) assayed for that line in that batch (block); same explanation for columns "Reproduced 27" and "Reproduced 29"

Batch = block number

inv columns are the inversion status of each line for each of the main inversions characterized in the DGRP; ST = standard, INV = inversion. ST/INV = unfixed status in the line

wolb = wolbachia status of each line; y = infected, n = not infected

Intercepts columns are the derived intercepts for each temperature using a random slopes model as described in the text

Slopes is the derived slope across temperatures using a random slopes model as described in the text


Tab: Canton S male data (= Zwoinska20_CSmaledata.csv)

This tab contains data from assaying Canton S used to account for variation across blocks. Table headers are explained below:

Batch = block

CScase25 = Canton S (CS) case (number of vials with at least one larva) at 25°C; same explanation for column "CScase29"

CStotal25 = Canton S (CS) total (total number of vials assayed) at 25°C; same explanation for column "CStotal29)

CSprop25 = CScase25/CStotal25; same explanation for "CSprop29"


Tab: Female data raw (= Zwoinska20_femalerawdata.csv)

This tab contains the raw data from assaying female fertility at three different temperatures for DGRP lines assigned as High or Low (see text for description) for male fertility performance across temperatures. Table headers are explained below:

Batch = block

DGRP = DGRP line

LH_code = whether the assayed line is considered to be a High (H) performing male line or a Low (L) performing male line

Temperature = developmental temperature experienced

Vial = vial where each pair was kept

Larvae = 1, if larvae present; 0, if no larvae present


Tab: Female data (= Zwoinska20_femaledata.csv)

This tab contains compacted data per line from the female fertility assay at three different temperatures for DGRP lines assigned as High or Low (see text for description) for male fertility performance across temperatures. Table headers are explained below:

Batch = block

LH_code = whether the assayed line is considered to be a High (H) performing male line or a Low (L) performing male line

DGRP = DGRP line

Temperature = developmental temperature experienced

case = number of vials with at least one larva

total = total number of vials (females) assayed


Tab: Canton S female data (= Zwoinska20_CSfemaledata.csv)

This tab contains data from assaying Canton S used to account for variation across blocks. Table headers are explained below:

Batch = block

CScase25 = Canton S (CS) case (number of vials with at least one larva) at 25°C; same explanation for column "CScase29"

CStotal25 = Canton S (CS) total (total number of vials assayed) at 25°C; same explanation for column "CStotal29)

CSprop25 = CScase25/CStotal25; same explanation for "CSprop29"