Fig. 2b-c | Quantitative immunofluorescence microscopy for intracellular FUS localization in ALS motor neurons

<p dir="ltr">This item is part of the <b>Figshare Project</b>:<br><b>Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution</b></p><p><br></p><p dir="ltr">From <i>Data Availability Statement</i> for the paper in <i>Nature Communications</i> entitled:</p><p dir="ltr"><b>Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS</b></p><p dir="ltr">"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The <i>C9orf72</i>-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.</p><p dir="ltr">[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."</p><p dir="ltr">-------------------------<br>[Item specific description:]</p><p><br></p><p dir="ltr">Quantititative fluorescene microscopy for FUS protein in ALS motor neurons</p><p dir="ltr">The dataset are Harmony archives for the high content imager Opera Phenix (Perkim Elmer). The proprietary harmony software creates a database with file organization stored in xml and oar files and folders with raw tif files. More information here on our imaging platform: <a href="https://www.kclwcic.co.uk/operaphenix" target="_blank">https://www.kclwcic.co.uk/operaphenix</a></p><p dir="ltr">These archives contain thousands of tif files. Therefore, they are compressed into tar.gz files with matching md5 checksums for verification.</p><p dir="ltr">The prism file is just a graphpad prism file with the summary data. More info here: <a href="https://www.graphpad.com/features" target="_blank">https://www.graphpad.com/features</a></p>