Fig. 2b-c | Quantitative immunofluorescence microscopy for intracellular FUS localization in ALS motor neurons

This item is part of the Figshare Project:
Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution

From Data Availability Statement for the forthcoming paper in Nature Communications entitled:

Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS

"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.

[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."

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[Item specific description:]

Quantititative fluorescene microscopy for FUS protein in ALS motor neurons

The dataset are Harmony archives for the high content imager Opera Phenix (Perkim Elmer). The proprietary harmony software creates a database with file organization stored in xml and oar files and folders with raw tif files. More information here on our imaging platform: https://www.kclwcic.co.uk/operaphenix

These archives contain thousands of tif files. Therefore, they are compressed into tar.gz files with matching md5 checksums for verification.

The prism file is just a graphpad prism file with the summary data. More info here: https://www.graphpad.com/features