Fig. 3d | TDP-43 localization and phosphorylation in ALS motor neurons

This item is part of the Figshare Project:
Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution

From Data Availability Statement for the forthcoming paper in Nature Communications entitled:

Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS

"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.

[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."

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[Item specific description:]

Intracellular localization of TARDBP in motor neurons. These files are confocal images of immunofluorescent stainings of TDP-43 and phosphorylated TDP-43 in iPSC-derived motor neurons. These files are confocal images of immunofluorescent stainings of TDP-43 and phosphorylated TDP-43 in iPSC-derived motor neurons. The file format is .czi, through the ZEN software (Zeiss). To view and process .czi files, Zeiss recommends using ImageJ and the ImageJ-based Fiji software package. Zeiss recommends using ImageJ and the ImageJ-based Fiji software package.

Information about the microscopy file format CZI and fluorescence threshold file format CZDSP can be found here: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.html