Fig. 3e-f | Quantitative immunofluorescence for TDP-43 localization in motor neuron lines

<p dir="ltr">This item is part of the <b>Figshare Project</b>:<br><a href="https://su.figshare.com/projects/Early_mitochondrial_dysfunction_revealed_across_FUS-_and_TARDBP-ALS_at_single_cell_resolution/163252" rel="noreferrer" target="_blank"><b>Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution</b></a></p><p><br></p><p dir="ltr">From <i>Data Availability Statement</i> for the forthcoming paper in <i>Nature Communications</i> entitled:</p><p dir="ltr"><b>Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS</b></p><p dir="ltr">"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The <i>C9orf72</i>-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.</p><p dir="ltr">[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."</p><p>--------------------------------------------</p><p dir="ltr">[Item specific description:]</p><p dir="ltr">Immunofluorescence microscopy of TDP-43 in in-vitro differentiated ALS motor neurons.</p><p dir="ltr">These files are related to quantification of TDP-43 localization and phosphorylation in ALS motor neurons using the Opera Phenix high content imaging system. More information here on our imaging platform: <a href="https://www.kclwcic.co.uk/operaphenix" target="_blank">https://www.kclwcic.co.uk/operaphenix</a></p><p dir="ltr">Sample meta information and plate layouts are available as XLSX and PDF files.</p><p dir="ltr">The file format is .czi, through the ZEN software (Zeiss). Zeiss recommends using ImageJ and the ImageJ-based <a href="https://imagej.net/software/fiji/" target="_blank">Fiji software package</a>.<br>Information about the microscopy file format CZI can be found here: <a href="https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.html" rel="noreferrer" target="_blank">https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.html</a></p><h2><br></h2><p><br></p>