Fig. 5m-n | Quantitative PCR to determine copy numbers of mitochondria in ALS motor neurons

<p dir="ltr">This item is part of the <b>Figshare Project</b>:<br><a href="https://su.figshare.com/projects/Early_mitochondrial_dysfunction_revealed_across_FUS-_and_TARDBP-ALS_at_single_cell_resolution/163252" rel="noreferrer" target="_blank"><b>Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution</b></a></p><p><br></p><p dir="ltr">From <i>Data Availability Statement</i> for the paper in <i>Nature Communications</i> entitled:</p><p dir="ltr"><b>Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS</b></p><p dir="ltr">"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The <i>C9orf72</i>-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.</p><p dir="ltr">[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."</p><p>--------------------------------------------</p><p dir="ltr">[Item specific description:]</p><p dir="ltr">Mitochondria genome and nuclear genome copy numbers were determined by qPCR.</p><p dir="ltr">These files relate to quantitative PCR to determine copy numbers of mitochondria in ALS motor neurons. The qPCR machine used was Applied Biosystems 6400 fast real-time PCR system and the files.</p><p dir="ltr">RNA was assessed by Nanodrop 2000 spectrometry. The spectra and concentration values were exported as NDV files, which are tab separated data tables.</p><p dir="ltr">Sample meta informations are saved as data tables in TXT and XLSX file format.</p><p dir="ltr">Raw experimental files from ABI 7500 Fast Real-Time PCR System (Applied Biosystems) are in the file format SDS. More information about the SDS file format can be found here: https://apps.thermofisher.com/apps/help/MAN0010504/GUID-0F46BF98-D779-4BB9-8008-EDA6792FA4DC.html</p><p dir="ltr">The melting curves and CT tables from qPCR analysis were exported as CSV files.</p><p><br></p>