Stockholm University
Browse

Fig. 6 | Overview of mitochondria in radially grown motor axons

Download (8.94 GB)
dataset
posted on 2025-04-24, 08:02 authored by Christoph SchweingruberChristoph Schweingruber

This item is part of the Figshare Project:
Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution


From Data Availability Statement for the forthcoming paper in Nature Communications entitled:

Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS

"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.

[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."

--------------------------------------------

[Item specific description:]

Motor neurons were specified from neuronal progenitors which were attached as embryoid body. Following maturation, the axons grew out radially. Mitochondria were labelled with TMRM before live cell microscopy.

These files are images and time-lapse videos of mitochondrial movement in human stem cell-derived motor axons based on TMRM-stained mitochondria. The file format is .czi, through the ZEN software (Zeiss). Zeiss recommends using ImageJ and the ImageJ-based Fiji software package.

Information about the microscopy file format CZI can be found here: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.html

History

Associated Publication

Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS. (forthcoming in: Nature Communications)

Affiliation (institution of first SU-affiliated author)

  • 431 Institutionen för biokemi och biofysik (DBB) | Department of Biochemistry and Biophysics (DBB)

access_level

  • public

access_condition

  • PUBLIC

Usage metrics

    Stockholm University

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC