Metagenomic NrdJm5 sequences placed in full phylogeny

To search for sequences from metagenomics projects, we downloaded all TARA Ocean ORFs (Eren 2017, ; Delmont 2018,, all ORFs from the Human Microbiome Project (2019-01-09; HMP 2012a, ; HMP 2012b, ) the majority of bacterial MAGs and SAGs from IMG/MER (4910 MAGs, 2230 SAGs) plus 53 aquatic and soil metagenomes, in particular those with project names containing “virus”, “phage”, “therm” or “hot“ (see img_sags.tsv, img_metag_samples.tsv and img_mags.tsv) (Markowitz 2008, Together, we downloaded a total of 250,881,638 ORFs. We used hmm profiles designed for each clan in the phylogeny to search the sequences. We found 181 sequences with a best match to the profile designed from the TV clan. These were aligned to the original alignment using Clustal Omega in profile mode ( ; Sievers 2014, and phylogenetically placed in the phylogeny from with RAxML (Stamatakis 2014, The resulting tree can be viewed with Dendroscope (Huson et al. 2007,; placed sequences have "QUERY" prepended to their names.