Rodrigues et al. Fluctuating heat stress data

https://doi.org/10.17045/sthlmuni.13211204

dataset

posted on 2021-11-09, 15:00 authored by Leonor RodriguesLeonor Rodrigues, Hayley A. McDermott, Irene Villanueva, Jana Djukarić, Lena C. Ruf, Mirjam AmcoffMirjam Amcoff, Rhonda R. Snook

Summary: This data was used to assess how fluctuating sublethal heat stress during development impacted subsequent male reproductive performance and success. Using a subset of isogenic lines of the Drosophila Genetic Reference Panel (DGRP) reared at standardized densities in four thermal conditions (Constant 25°C, Constant 29°C, Fluctuating 25°C, Fluctuating 29°C), we quantified phenotypic (and genetic) variation in mating propensity, fertility, productivity and sex-ratio. Files available: We provide 2 txt files: FLUCT_mating_fertility; FLUCT_productivity_sexratio. FLUCT_mating_fertility: This file contains the raw data of male mating propensity and fertility under four thermal conditions. Table headers are explained below: LineStatus - classification based on the reproductive performance across three temperatures tested in Zwoinska et al. (2020)*. (High – lines whose fertility did not decline as temperature increased; Low – lines whose fertility declined substantially as temperature increased) DGRP- line identity Wolbachia- line infection status (y – infected; n- uninfected) Haplotype- haplotypes identified in Bevers et al. (2019)* Inv1/2/7/8/9 - the inversion status of each line for each of the main inversions characterized in the DGRP (ST = standard, INV = inversion. ST/INV = unfixed status in the line) Temperature - mean developmental temperature experienced (25°C or 29°C) ThermalRegime - thermal variation experienced (Const - constant or Fluct - fluctuating regime) Vial – vial where each pair was kept EggCollDay – day the eggs were collected and placed in one of the thermal conditions. PairingDay – day males and females were paired Mating – mating propensity (1, if mating observed; 0, if no mating observed) Larvae – fertility (1, if larvae present; 0, if no larvae present) FLUCT_productivity_sexratio: This file contains the raw data of male productivity and sex ratio under four thermal conditions. Table headers are explained below: LineStatus - classification based on the reproductive performance across three temperatures tested in Zwoinska et al. 2020. (High - whose fertility did not decline as temperature increased; Low - whose fertility declined substantially as temperature increased) DGRP- line identity Wolbachia- line infection status (y – infected; n- uninfected) Haplotype- haplotypes identified in Bevers et al. (2019)* Inv1/2/7/8/9 - the inversion status of each line for each of the main inversions characterized in the DGRP (ST = standard, INV = inversion. ST/INV = unfixed status in the line) Temperature - mean developmental temperature experienced (25°C or 29°C) ThermalRegime - thermal variation experienced (Const - constant or Fluct - fluctuating regime) Vial – vial where each pair was kept EggCollDay – day the eggs were collected and placed in one of the thermal conditions. PairingDay – day males and females were paired Productivity – number of adult offspring produced. Daughters – number of female offspring produced. Sons – number of male offspring produced. IGV – absolute deviation from the median as a measure of intra-genotypic variability. *Bevers, R. P. J., et al (2019). Mitochondrial haplotypes affect metabolic phenotypes in the Drosophila Genetic Reference Panel. Nature Metabolism, 1, 1226–1242. <a href="https://doi.org/10.1038/s42255-019-0147-3">https://doi.org/10.1038/s42255-019-0147-3</a> Zwoinska, M. K., Rodrigues, L. R., Slate, J., & Snook, R. R. (2020). Phenotypic responses to and genetic architecture of sterility in response to sub-lethal temperature during development. Frontiers in Genetics, 11, 573. https://doi.org/10.3389/fgene.2020.00573