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Supplementary Fig. 9d-g | Metabolic profiling of ALS motor neurons

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posted on 2025-04-28, 17:27 authored by Christoph SchweingruberChristoph Schweingruber, Michaela Keuper, Martin Jastroch
<p dir="ltr">This item is part of the <b>Figshare Project</b>:<br><a href="https://su.figshare.com/projects/Early_mitochondrial_dysfunction_revealed_across_FUS-_and_TARDBP-ALS_at_single_cell_resolution/163252" rel="noreferrer" target="_blank"><b>Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution</b></a></p><p><br></p><p dir="ltr">From <i>Data Availability Statement</i> for the paper in <i>Nature Communications</i> entitled:</p><p dir="ltr"><b>Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS</b></p><p dir="ltr">"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The <i>C9orf72</i>-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.</p><p dir="ltr">[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."</p><p>--------------------------------------------</p><p dir="ltr">[Item specific description:]</p><p dir="ltr">Metabolic profiling of in-vitro differentiated ALS motor neurons using Seahorse metabolic flux measurements.</p><p dir="ltr">The raw experimental files generated with the Seahorse XF flux analyser (Agilent). are in the proprietary file format ASYR. More information about the ASYR file format can be found here: https://www.agilent.com/cs/pubimages/misc/ReadMe_Wave_Desktop_2-6.pdf</p>

History

Original language

  • English

Associated Publication

Schweingruber, C., Nijssen, J., Mechtersheimer, J. et al. (2025): Single-cell RNA-sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS. Nat Commun 16, 4633. https://doi.org/10.1038/s41467-025-59679-1

Affiliation (institution of first SU-affiliated author)

  • 431 Institutionen för biokemi och biofysik (DBB) | Department of Biochemistry and Biophysics (DBB)

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  • public

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  • PUBLIC

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